Cryopreservation of Shoot Tips of Selaginella uncinata
نویسندگان
چکیده
منابع مشابه
Cryopreservation of Potato Shoot Tips
Potato is one of the most important staple crops in both developed and developing countries of the world. Potato genetic resources are a prerequisite for potato breeding in both conventional and genetic engineering programmes. Cryopreservation has long been considered an ideal means for the long-term conservation of plant genetic resources. This review provides comprehensive information on vari...
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Vitrification refers to the physical process by which a liquid supercools to very low temperatures and finally solidifies into a metastable glass, without undergoing crystallization at a practical cooling rate. Thus, vitrification is an effective freeze-avoidance mechanism and living tissue cryopreservation is, in most cases, relying on it. As a glass is exceedingly viscous and stops all chemic...
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Shoot tips of cassava (Manihot esculenta Crantz) in vitro plantlets were successfully cryopreserved using the encapsulation-vitrification technique. Nodal cuttings of 5 mm length with one leaf were cultured on modified MS medium in Petri dishes (90 mm x 20 mm) for about 28 days. Excised shoot tips were precultured on sucrose enriched (0.3 M) medium for 16 h, encapsulated and osmoprotected with ...
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Shoot tips from 4to 6-week-old in vitro grown plantlets of six different cultivars of papaya were cryopreserved by vitrification. Shoot tips were treated in a 2 ml cryo-tube filled with a solution of 2 M glycerol and 0.4 M sucrose at 25°C for 20 min, then dehydrated with 1 ml pre-cooled vitrification solution at 4°C for 60 min. Treated samples were kept in liquid nitrogen for at least one week....
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ژورنال
عنوان ژورنال: American Fern Journal
سال: 2001
ISSN: 0002-8444,1938-422X
DOI: 10.1640/0002-8444(2001)091[0037:costos]2.0.co;2